Kwimimandla ye-biophapharceuticals kunye nophando lwezenzululwazi, ukususwa kwesayensi kunye nokugxila kwezinto ngabantu kubalulekile kuzo zombini iinkqubo zokuvavanywa nemveliso. Iindlela zendabuko ezinje nge-Evaporation kunye ne-Gentrigigation zihlala zinengxaki yokungasebenzi kakuhle, ukulahleka kwezithako ezisebenzayo, kunye nokususwa okungaphelelanga. I-labhoratri ihambisa i-worzers, kunye neenzuzo zabo ezikhethekileyo zeqondo lobushushu eliphantsi kunye noxinzelelo oluphantsi, ziye zavela njengezixhobo ezifanelekileyo kwezi nkqubo. Phakathi kwabo,"Zombini" ze-freezersziphambili ekuqhubeni le teknoloji.
Inzululwazi yesabangelwa kukukhululeka: Ukupheliswa kweqondo lokushisa eliphantsi
A I-Laboratrid Freefariphumelela ukususwa kwesolwvent kunye nokugxila kwezinto kwezinto ngeendlela ezintathu eziphambili:
Inqanaba laphambi kokubetha:Izinto ezinezixhobo eziqulathayo ziqhwanyiwe ngokukhawuleza kumaqondo obushushu asusela kwi--40 ° C ukuya kwi-30 ° C, akha amakristali omkhenkce.
Ukomisa okuqaqambileyo (ukuqaqamba):Phantsi kwendawo yevarvaum (eqhelekileyo ngezantsi kwe-10PA), iikristale zezomkhenkce ze-ice ngqo ngqo kwi-vapor yamanzi, isusa ngaphezulu kwe-90% ye-solvent.
Ukutyulwa kwesibini (Ukuthathwa):Iqondo lobushushu elithambileyo (20-40 ° C) liququzelele ukufuduka ngokupheleleyo kwamanzi, kukhokelela kwi-1% ye-5%.
Le nkqubo isusa umonakalo wobushushu obuphezulu kwizinto ezibuthathaka, igcina isakhiwo seemolekyuli zeproteni, i-ezymes, kunye neevithamini. Ukongeza, kudala isakhiwo esinentloko eququzelela ukubuyisela okulula ukuphuma kwehlelo okanye isicelo ngqo.
Izibonelelo eziphambili zokusebenza kwelebhu zangaphantsi
Xa kuthelekiswa nezixhobo ezizezimali, iilebhu ze-labhoratri ibonelela ngolawulo olungaphezulu lolawulo oluchanekileyo kunye nezibonelelo ezincinci ze-batch:
Ulawulo lobushushu obuchanekileyo:Imodeli "yoo" ye "Zlgj-12, umzekelo, isebenzisa itekhnoloji yokupholisa i-cinezela ye-cinezela, ukufikelela kumaqondo obushushu emiqolo asezantsi80° C ukuqinisekisa ukukhululeka ngokukhawuleza.
Ulawulo lweVenkile ekrelekrele:Izifo eziphezulu zokungalunganga ngokuqhubekayo zibeka iliso kumanqanaba evacuum (≤5PA), kunye neendlela zokhuseleko ezakhiweyo ukuthintela ukugcinwa kwesinyibilikisi.
Ukufudumeza ngokungxamisekileyo:Ixhotywe nge-helf efihlakeleyo yeshelf (njenge "zombini" i-Free Star i-tender yolawulo lwe-PLL), ezi nkqubo zivumela i-curgeserey elungiselelwe i-curves yesilinganiselo se-curting yezinto ezahlukeneyo, ekwakhetheka kakhulu.
Umzekelo, kuvavanyo lwelebhu yelebhu ebandakanya i-antidy-yomisa iindlela zokumisa, iindlela zemveli ezikhokelela ekuthinjweni zeprotheni kunye nokwenza izinto. Ngokwahlukileyo, ukomisa amaqondo obushushu asezantsi agcinwe ngaphezulu kwe-95% yomsebenzi we-antidy, kunye nomgubo obandayo ukuba uzinzile kwisitoreji sexesha elide.
Njengenkampani ekhethekileyo kwizixhobo ze-freeze-shreming yeminyaka eli-17 ubudala, "bobabini" boblensi baphucule ukususwa kwezeranti ngokuqhubekayo.
I-1. Inkqubo yokulandelela kwenkqubo epheleleyo
I "Zombini" zelebhu zelebhu zangaphantsi zixhotywe ngemodyuli yokugcina imibala kunye nemodyuli yokugcina idatha (ikwazi ukugcina iirekhodi ezingama-100,000). Inika ijikajika lexesha lokwenyani - ijikajika le-vacuum, ivumela abaphandi ukuba bamisele ngokuchanekileyo isiphelo seminqaba yeSolvent, ukuthintela imicimbi yokomisa ngaphezulu okanye imicimbi yokuyisiseko.
2. Iindlela ezininzi zokhuseleko loKhuseleko
I-copguague yempompo ye-Impofuum ukuba iqondo lobushushu le-trap lingaphezulu -50 ° C lokuthintela umonakalo wokubuyela umva.
Isandi kunye ne-alamu yokukhanya kunye noKhuseleko oluzenzekelayo kwimeko yokungaphumeleli okanye ukungabi nangqondo.
Inketho ye-UPSES ye-UPRORS igcina ukusebenza inkqubo yokulawula imizuzu engama-20 ngexesha lokuphuma kwamandla, kuqinisekisa ukuqhubeka kovavanyo.
3. Ulwando lomsebenzi wemodyuli
Ngenkqubo yenaliti yegesi ezenzekelayo, igesi ye-intert efana ne-nitrogen ingaziswa kwigumbi elomileyo lithintela ukuphendula kwe-oxidation (umz, e-Ethanol). Igumbi, elenziwe kwintsimbi engenasimo icocekileyo enomkhombandlela we-Diail-free yoyilo, isuse umngcipheko wokungcola ungcoliseko.
I-labhoratri ye-labhoratri i-freezers ivele kwizixhobo ezilula zokudiliza kwigumbi lokulawula inkqubo. "Zombini"Zlgjuthotho, Ngokwimo yayo ekrelekrele neyemongozi, ayingaphumi nje ukuphuculwa kwenkqubo yokususwa kweSolvent kuphela kwaye ikwanceda ukubeka iliso okwexeshana kunye nokusebenza kwexesha lokufumana inkqubo. Abaphandi ababeka phambili iziphumo zokulinga kunye nezihlulelo ezichazwe ngokuchanekileyo, ezi zixhobo zingumncedisi omiselweyo "omiselweyo" kwilebhu.
Ixesha lokuphumla: Mar-13 ukuya ku-525
