Kwimimandla ye-biopharmaceuticals kunye nophando lwezenzululwazi, ukususwa kwe-solvent kunye nokugxila kwizinto eziphathekayo ngamanyathelo abalulekileyo kuzo zombini iinkqubo zovavanyo kunye nemveliso. Iindlela zemveli ezifana nokuphuma komphunga kunye ne-centrifugation zihlala zihlupheka ngenxa yokungasebenzi kakuhle, ukulahleka kwezithako ezisebenzayo, kunye nokususwa kwe-solvent engaphelelanga. Izixhobo ezomisa umkhenkce zaseLabhoratri, ezinezibonelelo zazo ezizodwa zobushushu obuphantsi kunye noxinzelelo oluphantsi, ziye zavela njengezixhobo ezisebenza kakhulu kwezi nkqubo. Phakathi kwabo,"ZOMBINI" izomisi zomkhenkcebaphambili ekuqhubeleni phambili obu buchwepheshe.
ISayensi eNgemva komkhenkce-Ukomisa: Ukuphelelwa ngamanzi kubushushu obuphantsi
A ilabhoratri yomkhenkce isomisiifezekisa ukususwa kwe-solvent kunye nokugxininiswa kwezinto eziphathekayo ngezigaba ezintathu eziphambili:
Inqanaba lokukhenkceza kwangaphambili:Izinto eziqulethe i-solvents zikhenkcezwa ngokukhawuleza kumaqondo obushushu ukusuka kwi -40 ° C ukuya -80 ° C, zenza iikristale zeqhwa eziqinileyo.
Ukomisa okuPhambili (Ukuthotywa):Ngaphantsi kwendawo yokucoca (ngokuqhelekileyo ingaphantsi kwe-10Pa), iikristale zeqhwa zihamba ngokuthe ngqo kumphunga wamanzi, zisusa ngaphezu kwe-90% ye-solvent.
Ukomisa okwesibini (Ukuchithwa):Ukunyuka kobushushu obuphakathi (20-40 ° C) kunceda ukuchithwa ngokupheleleyo kwamanzi abophekileyo, okukhokelela kwisiqulatho sokufuma sokugqibela se-1% -5%.
Le nkqubo iphelisa umonakalo ophezulu wokushisa kwizinto ezithintela ukushisa, ukugcina isakhiwo se-molecular of proteins, i-enzymes, kunye neevithamini. Ukongeza, yenza i-porous structure eyenza lula ukubuyisela amanzi emanzini okanye ukusetyenziswa ngokuthe ngqo.
Izinto eziluncedo ezingundoqo kwiLabhoratri yokuMkhenkcisa izomisi
Xa kuthelekiswa nezixhobo zomgangatho wemizi-mveliso, izomisi zomkhenkce zaselabhoratri zibonelela ngolawulo oluchanekileyo oluphezulu kunye neenzuzo zokusetyenzwa kweebhetshi ezincinci:
Ulawulo lobushushu obuchanekileyo:"ZOMBINI" imodeli yokomisa umkhenkceZLGJ-12, umzekelo, isebenzisa iteknoloji yokupholisa icompressor evela kumazwe angaphandle, ifikelela kumaqondo obushushu aphantsi kangangoko -80°C ukuqinisekisa ukukhenkceza okukhawulezileyo.
Ulawulo lweVacuum olukrelekrele:Izinzwa eziphezulu ezichanekileyo zihlala zijonga amanqanaba e-vacuum (≤5Pa), kunye neendlela zokukhusela ezakhelwe ngaphakathi ukukhusela ukugcinwa kwe-solvent.
Ukufudumeza kweThanga lokuNxibelela:Ixhotyiswe ngenkqubo yokufudumeza kweshelufu ecwangcisiweyo (efana ne "BOTH" yokumisa itekhnoloji yokulawula i-PLD), ezi nkqubo zivumela amaqondo obushushu alungelelanisiweyo kwizinto ezahlukeneyo, ukwandisa ukugxila.
Umzekelo, kuvavanyo lwelabhoratri yebhayoloji ebandakanya ukomisa umkhenkce we-antibody, iindlela eziqhelekileyo zokuvela komphunga zikhokelele ekudityanisweni kweprotein kunye nokuyekisa ukusebenza. Ngokwahlukileyo, ukumisa umkhenkce kumaqondo obushushu aphantsi agcinwe ngaphezulu kwe-95% yomsebenzi we-antibody, kunye nomgubo obangelwayo uzinzile ngakumbi ukugcinwa kwexesha elide.
Njengenkampani egxile kwizixhobo zokomisa umkhenkce zasekhaya iminyaka eli-17, "BOTHINI" izomisi zokukhenkcisa ziye zaphucula ukusebenza kakuhle kokususa isinyibilikisi ngokuqhubekeka kokutsha kwetekhnoloji.
1. Inkqubo egcweleyo yokuLandelwa kweDatha
I-"BOTH" i-laboratory freeze dryer ixhotywe ngombala wesikrini kunye nemodyuli yokugcina idatha (ekwazi ukugcina iirekhodi ze-100,000). Inika i-real-time heat-vacuum curves, eyenza abaphandi baqonde ngokuchanekileyo isiphelo se-solvent sublimation, ukuthintela ukomisa kakhulu okanye imiba ye-solvent eseleyo.
2. IiNdlela ezininzi zoKhuseleko loKhuseleko
Ukuvalwa kwempompo yokucoca okuzenzekelayo ukuba ubushushu bomgibe bungaphezulu kwe -50°C ukunqanda umonakalo obuya ngasemva.
Isandi kunye ne-alam ezikhanyayo kunye nokusetyenziswa kokhuseleko oluzenzekelayo kwimeko yokusilela kwe-vacuum okanye ukuguquguquka okungaqhelekanga kobushushu.
Unikezelo lwamandla okuzithandela lwe-UPS lugcina ukusebenza kwenkqubo yokulawula imizuzu engama-20 ngexesha lokuphela kombane, ukuqinisekisa ukuqhubeka kovavanyo.
3. Ukwandiswa koMsebenzi weModyuli
Ngenkqubo yokutofa kwegesi ezenzekelayo ezenzekelayo, igesi engasebenziyo njenge-nitrogen ingangeniswa kwigumbi lokumisa ukuthintela ukusabela kwe-oxidation ngexesha loxinaniso lwezinyibilikisi eziziva ngeoksijini (umzekelo, i-ethanol). Igumbi, elenziwe kwintsimbi ecocekileyo ecocekileyo ene-coil-free trap design, isusa imingcipheko yokungcola.
Izixhobo zokomisa kwilabhoratri zomkhenkce ziye zavela kwizixhobo ezilula zokuphelelwa ngamanzi emzimbeni ukuya kumaqonga olawulo lwenkqubo entsonkothileyo. I "ZOBINI"ZLGJuthotho, ngoyilo lwayo olukrelekrele kunye nemodyuli, ayiphuculi kuphela ukususwa kwe-solvent kodwa ikwenza kube lula ukujonga ixesha lokwenyani kunye nokulungiswa kwenkqubo yoxinaniso. Kubaphandi ababeka phambili ukuchaneka kovavanyo kunye neziphumo ezizinzileyo, ezi zixhobo ziba "ngumncedisi wenkqubo omiselweyo" oyimfuneko kwiilabhoratri.
Ixesha lokuposa: Mar-13-2025
